5F9R: Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage

PDB ID: 5F9RDownload
MMDB ID: 136058
PDB Deposition Date: 2015/12/10
Updated in MMDB: 2019/11
Experimental Method:
x-ray diffraction
Resolution: 3.4  Å
Source Organism:
Streptococcus pyogenes serotype M1▼
Similar Structures:
Biological Unit for 5F9R: tetrameric; determined by author and by software (PISA)
Molecular Components in 5F9R
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease Cas9/csn1
Molecule annotation
Nucleotides(3 molecules)
1
RNA (116-mer)
Molecule annotation
1
DNA (30-mer)
Molecule annotation
1
DNA (5'-d(p*ap*tp*gp*ap*gp*ap*cp*gp*cp*tp*gp*gp*ap*gp*tp*ap*cp*ap*c)-3')
Molecule annotation
Chemicals and Non-standard biopolymers (3 molecules)
1
3
SULFATE IONSULFATE ION
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Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303