5F90: Crystal Structure Of A Crenomytilus Grayanus Lectin In Complex With Gb3 Allyl

Citation:
Abstract
In this study, we report the structure and function of a lectin from the sea mollusk Crenomytilus grayanus collected from the sublittoral zone of Peter the Great Bay of the Sea of Japan. The crystal structure of C. grayanus lectin (CGL) was solved to a resolution of 1.08 A, revealing a beta-trefoil fold that dimerizes into a dumbbell-shaped quaternary structure. Analysis of the crystal CGL structures bound to galactose, galactosamine, and globotriose Gb3 indicated that each CGL can bind three ligands through a carbohydrate-binding motif involving an extensive histidine- and water-mediated hydrogen bond network. CGL binding to Gb3 is further enhanced by additional side-chain-mediated hydrogen bonds in each of the three ligand-binding sites. NMR titrations revealed that the three binding sites have distinct microscopic affinities toward galactose and galactosamine. Cell viability assays showed that CGL recognizes Gb3 on the surface of breast cancer cells, leading to cell death. Our findings suggest the use of this lectin in cancer diagnosis and treatment.
PDB ID: 5F90Download
MMDB ID: 138127
PDB Deposition Date: 2015/12/9
Updated in MMDB: 2016/06
Experimental Method:
x-ray diffraction
Resolution: 1.64  Å
Source Organism:
Similar Structures:
Biological Unit for 5F90: dimeric; determined by author and by software (PISA)
Molecular Components in 5F90
Label Count Molecule
Proteins (2 molecules)
2
Galnac/gal-specific Lectin
Molecule annotation
Chemicals (17 molecules)
1
1
2
5
3
5
4
3
5
3
* Click molecule labels to explore molecular sequence information.

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