5EUZ: Rat prestin STAS domain in complex with iodide

Citation:
Abstract
Prestin is a unique ATP- and Ca(2+)-independent molecular motor with piezoelectric characteristics responsible for the electromotile properties of mammalian cochlear outer hair cells, i.e. the capacity of these cells to modify their length in response to electric stimuli. This 'electromotility' is at the basis of the exceptional sensitivity and frequency selectivity distinctive of mammals. Prestin belongs to the SLC26 (solute carrier 26) family of anion transporters and needs anions to function properly, particularly Cl(-). In the present study, using X-ray crystallography we reveal that the STAS (sulfate transporter and anti-sigma factor antagonist) domain of mammalian prestin, considered an 'incomplete' transporter, harbours an unanticipated anion-binding site. In parallel, we present the first crystal structure of a prestin STAS domain from a non-mammalian vertebrate prestin (chicken) that behaves as a 'full' transporter. Notably, in chicken STAS, the anion-binding site is lacking because of a local structural rearrangement, indicating that the presence of the STAS anion-binding site is exclusive to mammalian prestin.
PDB ID: 5EUZDownload
MMDB ID: 134914
PDB Deposition Date: 2015/11/19
Updated in MMDB: 2015/12
Experimental Method:
x-ray diffraction
Resolution: 2.403  Å
Source Organism:
Similar Structures:
Biological Unit for 5EUZ: monomeric; determined by author and by software (PISA)
Molecular Components in 5EUZ
Label Count Molecule
Protein (1 molecule)
1
Prestin,prestin(Gene symbol: Slc26a5)
Molecule annotation
Chemicals (12 molecules)
1
4
2
5
3
1
4
2
* Click molecule labels to explore molecular sequence information.

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