5ER0: Water-forming Nadh Oxidase From Lactobacillus Brevis (lbnox)

Citation:
Abstract
A decline in electron transport chain (ETC) activity is associated with many human diseases. Although diminished mitochondrial adenosine triphosphate production is recognized as a source of pathology, the contribution of the associated reduction in the ratio of the amount of oxidized nicotinamide adenine dinucleotide (NAD(+)) to that of its reduced form (NADH) is less clear. We used a water-forming NADH oxidase from Lactobacillus brevis (LbNOX) as a genetic tool for inducing a compartment-specific increase of the NAD(+)/NADH ratio in human cells. We used LbNOX to demonstrate the dependence of key metabolic fluxes, gluconeogenesis, and signaling on the cytosolic or mitochondrial NAD(+)/NADH ratios. Expression of LbNOX in the cytosol or mitochondria ameliorated proliferative and metabolic defects caused by an impaired ETC. The results underscore the role of reductive stress in mitochondrial pathogenesis and demonstrate the utility of targeted LbNOX for direct, compartment-specific manipulation of redox state.
PDB ID: 5ER0Download
MMDB ID: 138373
PDB Deposition Date: 2015/11/13
Updated in MMDB: 2017/10
Experimental Method:
x-ray diffraction
Resolution: 2.41  Å
Source Organism:
Similar Structures:
Biological Unit for 5ER0: dimeric; determined by author and by software (PISA)
Molecular Components in 5ER0
Label Count Molecule
Proteins (2 molecules)
2
Nadh Oxidase
Molecule annotation
Chemicals (11 molecules)
1
2
2
2
3
7
* Click molecule labels to explore molecular sequence information.

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