5EME: Complex Of Rna R(gcagcagc) With Antisense Pna P(ctgctgc)

PNA is a promising molecule for antisense therapy of trinucleotide repeat disorders. We present the first crystal structures of RNA-PNA duplexes. They contain CUG repeats, relevant to myotonic dystrophy type I, and CAG repeats associated with poly-glutamine diseases. We also report the first PNA-PNA duplex containing mismatches. A comparison of the PNA homoduplex and the PNA-RNA heteroduplexes reveals PNA's intrinsic structural properties, shedding light on its reported sequence selectivity or intolerance of mismatches when it interacts with nucleic acids. PNA has a much lower helical twist than RNA and the resulting duplex has an intermediate conformation. PNA retains its overall conformation while locally there is much disorder, especially peptide bond flipping. In addition to the Watson-Crick pairing, the structures contain interesting interactions between the RNA's phosphate groups and the Pi electrons of the peptide bonds in PNA.
PDB ID: 5EMEDownload
MMDB ID: 135513
PDB Deposition Date: 2015/11/6
Updated in MMDB: 2017/05
Experimental Method:
x-ray diffraction
Resolution: 1.15  Å
Source Organism:
Biological Unit for 5EME: dimeric; determined by author and by software (PISA)
Molecular Components in 5EME
Label Count Molecule
Nucleotides(2 molecules)
RNA (5'-r(*gp*cp*ap*gp*cp*ap*gp*c)-3')
Molecule annotation
Antisense PNA Strand
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB