5E8L: Crystal Structure Of Geranylgeranyl Pyrophosphate Synthase 11 From Arabidopsis Thaliana

Citation:
Abstract
Terpenoids are the largest and most diverse class of plant specialized metabolites, which function in diverse physiological processes during plant development. In the biosynthesis of plant terpenoids, short-chain prenyltransferase (SC-PT), together with terpene synthase (TPS), play a role in determining terpenoid diversity. SC-PTs biosynthesize prenyl pyrophosphates with different chain lengths and these compounds are the direct precursors of terpenoids. Arabidopsis thaliana possesses a subgroup of SC-PTs whose functions are not clearly known (we focus on 10 geranylgeranyl pyrophosphate synthase-like (GGPPSL) proteins here, which are commonly thought to produce GGPP [C20]). In this study, we find that a subset members of the Arabidopsis GGPPSL gene family have undergone neo-functionalization: GGPPSL6, 7, 9, and 10 mainly have geranylfarnesyl pyrophosphate synthase activity ([C25]; renamed as AtGFPPS1, 2, 3 and 4), and GGPPSL8 produces even longer chain prenyl pyrophosphate (>/= C30, renamed as polyprenyl pyrophosphate synthase 2, AtPPPS2). By solving the crystal structures of AtGFPPS2, AtPPPS2 and AtGGPPS11, we reveal the product chain-length determination mechanism of SC-PTs and describe it as a "three floors" model. Using this model, we identified a novel GFPPS clade distributed in Brassicaceae plants. In addition, we find that the GFPPS gene typically occurs in tandem with a gene encoding a TPS, forming a GFPPS-TPS gene cluster.
PDB ID: 5E8LDownload
MMDB ID: 134159
PDB Deposition Date: 2015/10/14
Updated in MMDB: 2015/11
Experimental Method:
x-ray diffraction
Resolution: 2.81  Å
Source Organism:
Similar Structures:
Biological Unit for 5E8L: dimeric; determined by author and by software (PISA)
Molecular Components in 5E8L
Label Count Molecule
Proteins (2 molecules)
2
Heterodimeric Geranylgeranyl Pyrophosphate Synthase Large Subunit 1, Chloroplastic(Gene symbol: GGPS1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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