5E2B: Crystal Structure Of Ntmt1 In Complex With N-terminally Methylated Ppkria Peptide

alpha-N-terminal methylation represents a highly conserved and prevalent post-translational modification, yet its biological function has remained largely speculative. The recent discovery of alpha-N-terminal methyltransferase 1 (NTMT1) and its physiological substrates propels the elucidation of a general role of alpha-N-terminal methylation in mediating DNA-binding ability of the modified proteins. The phenotypes, observed from both NTMT1 knockdown in breast cancer cell lines and knockout mouse models, suggest the potential involvement of alpha-N-terminal methylation in DNA damage response and cancer development. In this study, we report the first crystal structures of human NTMT1 in complex with cofactor S-adenosyl-L-homocysteine (SAH) and six substrate peptides, respectively, and reveal that NTMT1 contains two characteristic structural elements (a beta hairpin and an N-terminal extension) that contribute to its substrate specificity. Our complex structures, coupled with mutagenesis, binding, and enzymatic studies, also present the key elements involved in locking the consensus substrate motif XPK (X indicates any residue type other than D/E) into the catalytic pocket for alpha-N-terminal methylation and explain why NTMT1 prefers an XPK sequence motif. We propose a catalytic mechanism for alpha-N-terminal methylation. Overall, this study gives us the first glimpse of the molecular mechanism of alpha-N-terminal methylation and potentially contributes to the advent of therapeutic agents for human diseases associated with deregulated alpha-N-terminal methylation.
PDB ID: 5E2BDownload
MMDB ID: 133796
PDB Deposition Date: 2015/9/30
Updated in MMDB: 2015/12
Experimental Method:
x-ray diffraction
Resolution: 1.95  Å
Source Organism:
Similar Structures:
Biological Unit for 5E2B: dimeric; determined by software (PISA)
Molecular Components in 5E2B
Label Count Molecule
Proteins (2 molecules)
N-terminal Xaa-pro-lys N-methyltransferase 1(Gene symbol: NTMT1)
Molecule annotation
Molecule annotation
Chemicals (30 molecules)
Molecule information is not avaliable.
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Citing MMDB