5DWW: Structural Insights Into The Quadruplex-duplex 3' Interface Formed From A Telomeric Repeat - Ttloop

Citation:
Abstract
We report here on an X-ray crystallographic and molecular modeling investigation into the complex 3' interface formed between putative parallel stranded G-quadruplexes and a duplex DNA sequence constructed from the human telomeric repeat sequence TTAGGG. Our crystallographic approach provides a detailed snapshot of a telomeric 3' quadruplex-duplex junction: a junction that appears to have the potential to form a unique molecular target for small molecule binding and interference with telomere-related functions. This unique target is particularly relevant as current high-affinity compounds that bind putative G-quadruplex forming sequences only rarely have a high degree of selectivity for a particular quadruplex. Here DNA junctions were assembled using different putative quadruplex-forming scaffolds linked at the 3' end to a telomeric duplex sequence and annealed to a complementary strand. We successfully generated a series of G-quadruplex-duplex containing crystals, both alone and in the presence of ligands. The structures demonstrate the formation of a parallel folded G-quadruplex and a B-form duplex DNA stacked coaxially. Most strikingly, structural data reveals the consistent formation of a TAT triad platform between the two motifs. This triad allows for a continuous stack of bases to link the quadruplex motif with the duplex region. For these crystal structures formed in the absence of ligands, the TAT triad interface occludes ligand binding at the 3' quadruplex-duplex interface, in agreement with in silico docking predictions. However, with the rearrangement of a single nucleotide, a stable pocket can be produced, thus providing an opportunity for the binding of selective molecules at the interface.
PDB ID: 5DWWDownload
MMDB ID: 135762
PDB Deposition Date: 2015/9/23
Updated in MMDB: 2016/02
Experimental Method:
x-ray diffraction
Resolution: 2.79  Å
Source Organism:
Biological Unit for 5DWW: dimeric; determined by author
Molecular Components in 5DWW
Label Count Molecule
Nucleotides(2 molecules)
1
DNA (25-mer)
Molecule annotation
1
DNA (5'-d(*tp*ap*ap*cp*gp*cp*tp*a)-3')
Molecule annotation
Chemicals (3 molecules)
1
3
* Click molecule labels to explore molecular sequence information.

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