5DUR: Influenza A Virus H5 Hemagglutinin Globular Head In Complex With Antibody 100f4

Citation:
Abstract
Understanding the mechanism of protective antibody recognition against highly pathogenic avian influenza A virus H5N1 in humans is critical for the development of effective therapies and vaccines. Here we report the crystal structure of three H5-specific human monoclonal antibodies bound to the globular head of hemagglutinin (HA) with distinct epitope specificities, neutralization potencies and breadth. A structural and functional analysis of these epitopes combined with those reported elsewhere identifies four major vulnerable sites on the globular head of H5N1 HA. Chimeric and vulnerable site-specific mutant pseudoviruses are generated to delineate broad neutralization specificities of convalescent sera from two individuals who recovered from the infection with H5N1 virus. Our results show that the four vulnerable sites on the globular head rather than the stem region are the major neutralizing targets, suggesting that during natural H5N1 infection neutralizing antibodies against the globular head work in concert to provide protective antibody-mediated immunity.
PDB ID: 5DURDownload
MMDB ID: 134888
PDB Deposition Date: 2015/9/20
Updated in MMDB: 2015/12
Experimental Method:
x-ray diffraction
Resolution: 2.82  Å
Source Organism:
Influenza A virus (A/Anhui/1/2005(H5N1))
Similar Structures:
Biological Unit for 5DUR: hexameric; determined by author and by software (PISA)
Molecular Components in 5DUR
Label Count Molecule
Proteins (6 molecules)
2
Heavy Chain of Antibody 100f4
Molecule annotation
2
Light Chain of Antibody 100f4
Molecule annotation
2
Hemagglutinin
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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