5D9O: Crystal Structure Of Pbgh5a, A Glycoside Hydrolase Family 5 Enzyme From Prevotella Bryantii B14, E280a Mutant In Complex With Cellotetraose

Citation:
Abstract
The recent classification of Glycoside Hydrolase Family 5 (GH5) members into subfamilies enhances the prediction of substrate specificity by phylogenetic analysis. However, the small number of well-characterized members is a current limitation to understanding the molecular basis of the diverse specificity observed across individual GH5 subfamilies. GH5 Subfamily 4 (GH5_4) is one of the largest, with known activities comprising (carboxymethyl)cellulases, mixed-linkage endo-glucanases, and endo-xyloglucanases. Through detailed structure-function analysis, we have revisited the characterization of a classic GH5_4 carboxymethylcellulase, PbGH5A (also known as Orf4, CMCase, and Cel5A) from the symbiotic rumen Bacteroidetes Prevotella bryantii B14. We demonstrate that CMC and phosphoric acid-swollen cellulose (PASC) are in fact strikingly poor substrates for PbGH5A, which instead exhibits clear primary specificity for the plant storage and cell wall polysaccharide, mixed-linkage beta-glucan. Significant activity toward the plant cell wall polysaccharide xyloglucan was also observed. Determination of PbGH5A crystal structures in the apo form and in complex with (xylo)glucan oligosaccharides and an active-site affinity label, together with detailed kinetic analysis using a variety of well-defined oligosaccharide substrates, revealed the structural determinants of polysaccharide substrate specificity. In particular, this analysis highlighted the PbGH5A active-site motifs which engender predominant mixed-linkage endo-glucanase activity vis-a-vis predominant endo-xyloglucanases in GH5_4. However the detailed phylogenetic analysis of GH5_4 members did not delineate particular clades of enzymes sharing these sequence motifs; the phylogeny was instead dominated by bacterial taxonomy. Nonetheless, our results provide key enzyme functional and structural reference data for future bioinformatics analyses of meta(genomes) to elucidate the biology of complex gut ecosystems.
PDB ID: 5D9ODownload
MMDB ID: 133947
PDB Deposition Date: 2015/8/18
Updated in MMDB: 2015/11
Experimental Method:
x-ray diffraction
Resolution: 1.55  Å
Source Organism:
Similar Structures:
Biological Unit for 5D9O: monomeric; determined by author
Molecular Components in 5D9O
Label Count Molecule
Protein (1 molecule)
1
B-1,4-endoglucanase
Molecule annotation
Chemicals (6 molecules)
1
4
2
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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