5D2Q: Crystal Structure Of Stpr From Bombyx Mori In Complex With 20-bp Dna Derived From +290 Site Of The Fibroin Gene

Citation:
Abstract
Despite over 3300 protein-DNA complex structures have been reported in the past decades, there remain some unknown recognition patterns between protein and target DNA. The silkgland-specific transcription factor FMBP-1 from the silkworm Bombyx mori contains a unique DNA-binding domain of four tandem STPRs, namely the score and three amino acid peptide repeats. Here we report three structures of this STPR domain (termed BmSTPR) in complex with DNA of various lengths. In the presence of target DNA, BmSTPR adopts a zig-zag structure of three or four tandem alpha-helices that run along the major groove of DNA. Structural analyses combined with binding assays indicate BmSTPR prefers the AT-rich sequences, with each alpha-helix covering a DNA sequence of 4 bp. The successive AT-rich DNAs adopt a wider major groove, which is in complementary in shape and size to the tandem alpha-helices of BmSTPR. Substitutions of DNA sequences and affinity comparison further prove that BmSTPR recognizes the major groove mainly via shape readout. Multiple-sequence alignment suggests this unique DNA-binding pattern should be highly conserved for the STPR domain containing proteins which are widespread in animals. Together, our findings provide structural insights into the specific interactions between a novel DNA-binding protein and a unique deformed B-DNA.
PDB ID: 5D2QDownload
MMDB ID: 137689
PDB Deposition Date: 2015/8/6
Updated in MMDB: 2016/03
Experimental Method:
x-ray diffraction
Resolution: 2.4  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 5D2Q: dimeric; determined by author and by software (PISA)
Molecular Components in 5D2Q
Label Count Molecule
Protein (1 molecule)
1
Fibroin-modulator-binding Protein-1
Molecule annotation
Nucleotide(1 molecule)
1
DNA (40-mer)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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