5CXB: Structure Of Ytm1 Bound To The C-terminal Domain Of Erb1 In P21 21 2 Space Group

Citation:
Abstract
Ribosome biogenesis is one of the most essential pathways in eukaryotes although it is still not fully characterized. Given the importance of this process in proliferating cells, it is obvious that understanding the macromolecular details of the interactions that take place between the assembly factors, ribosomal proteins and nascent pre-rRNAs is essentially required for the development of new non-genotoxic treatments for cancer. Herein, we have studied the association between the WD40-repeat domains of Erb1 and Ytm1 proteins. These are essential factors for the biogenesis of 60S ribosomal subunits in eukaryotes that form a heterotrimeric complex together with the also essential Nop7 protein. We provide the crystal structure of a dimer formed by the C-terminal part of Erb1 and Ytm1 from Chaetomium thermophilum at 2.1 A resolution. Using a multidisciplinary approach we show that the beta-propeller domains of these proteins interact in a novel manner that leads to a high-affinity binding. We prove that a point mutation within the interface of the complex impairs the interaction between the two proteins and negatively affects growth and ribosome production in yeast. Our study suggests insights into the association of the Erb1-Ytm1 dimer with pre-ribosomal particles.
PDB ID: 5CXBDownload
MMDB ID: 133939
PDB Deposition Date: 2015/7/28
Updated in MMDB: 2015/12
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 5CXB: dimeric; determined by author and by software (PISA)
Molecular Components in 5CXB
Label Count Molecule
Proteins (2 molecules)
1
Ribosome Biogenesis Protein Ytm1(Gene symbol: CTHT_0061460)
Molecule annotation
1
Ribosome Biogenesis Protein Erb1(Gene symbol: CTHT_0057570)
Molecule annotation
Chemicals (7 molecules)
1
3
2
1
3
2
4
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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