5CUO: Structure of Rhodopseudomonas palustris PduL - CoA bound form

Citation:
Abstract
Bacterial Microcompartments (BMCs) are proteinaceous organelles that encapsulate critical segments of autotrophic and heterotrophic metabolic pathways; they are functionally diverse and are found across 23 different phyla. The majority of catabolic BMCs (metabolosomes) compartmentalize a common core of enzymes to metabolize compounds via a toxic and/or volatile aldehyde intermediate. The core enzyme phosphotransacylase (PTAC) recycles Coenzyme A and generates an acyl phosphate that can serve as an energy source. The PTAC predominantly associated with metabolosomes (PduL) has no sequence homology to the PTAC ubiquitous among fermentative bacteria (Pta). Here, we report two high-resolution PduL crystal structures with bound substrates. The PduL fold is unrelated to that of Pta; it contains a dimetal active site involved in a catalytic mechanism distinct from that of the housekeeping PTAC. Accordingly, PduL and Pta exemplify functional, but not structural, convergent evolution. The PduL structure, in the context of the catalytic core, completes our understanding of the structural basis of cofactor recycling in the metabolosome lumen.
PDB ID: 5CUODownload
MMDB ID: 137673
PDB Deposition Date: 2015/7/24
Updated in MMDB: 2018/06
Experimental Method:
x-ray diffraction
Resolution: 1.544  Å
Source Organism:
Similar Structures:
Biological Unit for 5CUO: dimeric; determined by author and by software (PISA)
Molecular Components in 5CUO
Label Count Molecule
Proteins (2 molecules)
2
Phosphate Propanoyltransferase
Molecule annotation
Chemicals (6 molecules)
1
2
2
4
* Click molecule labels to explore molecular sequence information.

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