5COM: Crystal Structure Of Uncharacterized Protein Q187f5 From Clostridium Difficile 630

Bacteria have developed a variety of mechanisms for surviving harsh environmental conditions, nutrient stress and overpopulation. Paenibacillus dendritiformis produces a lethal protein (Slf) that is able to induce cell death in neighbouring colonies and a phenotypic switch in more distant ones. Slf is derived from the secreted precursor protein, DfsB, after proteolytic processing. Here, we present new crystal structures of DfsB homologues from a variety of bacterial species and a surprising version present in the yeast Saccharomyces cerevisiae. Adopting a four-helix bundle decorated with a further three short helices within intervening loops, DfsB belongs to a non-enzymatic class of the DinB fold. The structure suggests that the biologically active Slf fragment may possess a C-terminal helix rich in basic and aromatic residues that suggest a functional mechanism akin to that for cationic antimicrobial peptides.
PDB ID: 5COMDownload
MMDB ID: 136209
PDB Deposition Date: 2015/7/20
Updated in MMDB: 2016/03
Experimental Method:
x-ray diffraction
Resolution: 1.85  Å
Source Organism:
Similar Structures:
Biological Unit for 5COM: monomeric; determined by author and by software (PISA)
Molecular Components in 5COM
Label Count Molecule
Protein (1 molecule)
Putative Conjugative Transposon Protein Tn1549-like, Ctn5- Orf2
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB