5BX2: Crystal Structure Of Thermoanaerobacterium Xylanolyticum Gh116 Beta- Glucosidase With 2-deoxy-2-fluoroglucoside

Citation:
Abstract
Human glucosylcerebrosidase 2 (GBA2) of the CAZy family GH116 is responsible for the breakdown of glycosphingolipids on the cytoplasmic face of the endoplasmic reticulum and Golgi apparatus. Genetic defects in GBA2 result in spastic paraplegia and cerebellar ataxia, while cross-talk between GBA2 and GBA1 glucosylceramidases may affect Gaucher disease. Here, we report the first three-dimensional structure for any GH116 enzyme, Thermoanaerobacterium xylanolyticum TxGH116 beta-glucosidase, alone and in complex with diverse ligands. These structures allow identification of the glucoside binding and active site residues, which are shown to be conserved with GBA2. Mutagenic analysis of TxGH116 and structural modeling of GBA2 provide a detailed structural and functional rationale for pathogenic missense mutations of GBA2.
PDB ID: 5BX2Download
MMDB ID: 139280
PDB Deposition Date: 2015/6/8
Updated in MMDB: 2016/08
Experimental Method:
x-ray diffraction
Resolution: 1.61  Å
Source Organism:
Similar Structures:
Biological Unit for 5BX2: monomeric; determined by author
Molecular Components in 5BX2
Label Count Molecule
Protein (1 molecule)
1
Beta-glucosidase
Molecule annotation
Chemicals (20 molecules)
1
1
2
17
3
1
4
1
* Click molecule labels to explore molecular sequence information.

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