5B2T: Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM)

PDB ID: 5B2TDownload
MMDB ID: 137665
PDB Deposition Date: 2016/2/2
Updated in MMDB: 2023/11
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Streptococcus pyogenes serotype M1▼
Similar Structures:
Biological Unit for 5B2T: tetrameric; determined by author and by software (PISA)
Molecular Components in 5B2T
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease Cas9
Molecule annotation
Nucleotides(3 molecules)
1
Guide RNA
Molecule annotation
1
Target DNA
Molecule annotation
1
Non-target DNA, DNA (5'-d(*tp*gp*cp*gp*ap*tp*tp*g)-3')
Molecule annotation
Chemicals and Non-standard biopolymers (19 molecules)
1
10
POTASSIUM IONPOTASSIUM ION
2
3
MAGNESIUM IONMAGNESIUM ION
3
5
1,2-ETHANEDIOL1,2-ETHANEDIOL
4
1
ACETATE IONACETATE ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303