5B2P: Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGA PAM)

PDB ID: 5B2PDownload
MMDB ID: 136965
PDB Deposition Date: 2016/2/1
Updated in MMDB: 2017/10
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
Francisella tularensis subsp. novicida U112▼
Similar Structures:
Biological Unit for 5B2P: tetrameric; determined by author and by software (PISA)
Molecular Components in 5B2P
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease Cas9
Molecule annotation
Nucleotides(2 molecules)
1
Target DNA
Molecule annotation
1
DNA (5'-d(*tp*gp*ap*tp*ap*tp*cp*gp*g)-3')
Molecule annotation
Chemicals and Non-standard biopolymers (50 molecules)
B
1
Guide RNA
1
1
ZINC IONZINC ION
2
4
SODIUM IONSODIUM ION
3
2
CHLORIDE IONCHLORIDE ION
4
17
CALCIUM IONCALCIUM ION
5
23
1,2-ETHANEDIOL1,2-ETHANEDIOL
6
2
ACETATE IONACETATE ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303