5AOX: Human Alu RNA retrotransposition complex in the ribosome-stalling conformation

Citation:
Abstract
The Alu element is the most successful human genomic parasite affecting development and causing disease. It originated as a retrotransposon during early primate evolution of the gene encoding the signal recognition particle (SRP) RNA. We defined a minimal Alu RNA sufficient for effective retrotransposition and determined a high-resolution structure of its complex with the SRP9/14 proteins. The RNA adopts a compact, closed conformation that matches the envelope of the SRP Alu domain in the ribosomal translation elongation factor-binding site. Conserved structural elements in SRP RNAs support an ancient function of the closed conformation that predates SRP9/14. Structure-based mutagenesis shows that retrotransposition requires the closed conformation of the Alu ribonucleoprotein particle and is consistent with the recognition of stalled ribosomes. We propose that ribosome stalling is a common cause for the cis-preference of the mammalian L1 retrotransposon and for the efficiency of the Alu RNA in hijacking nascent L1 reverse transcriptase.
PDB ID: 5AOXDownload
MMDB ID: 134079
PDB Deposition Date: 2015/9/12
Updated in MMDB: 2015/12
Experimental Method:
x-ray diffraction
Resolution: 2.04  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 5AOX: trimeric; determined by author and by software (PISA)
Molecular Components in 5AOX
Label Count Molecule
Proteins (2 molecules)
1
Signal Recognition Particle 9 KDA Protein(Gene symbol: SRP9)
Molecule annotation
1
Signal Recognition Particle 14 KDA Protein(Gene symbol: SRP14)
Molecule annotation
Nucleotide(1 molecule)
2
ALU JO Consensus RNA
Molecule annotation
Chemicals (6 molecules)
1
1
2
1
3
1
4
3
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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