5AMW: Crystal Structure Of The Strawberry Pathogenesis-related 10 (pr-10) Fra A 2 Protein (a141f) Processed With The Crystaldirect Automated Mounting And Cryo-cooling Technology

Citation:
Abstract
Currently, macromolecular crystallography projects often require the use of highly automated facilities for crystallization and X-ray data collection. However, crystal harvesting and processing largely depend on manual operations. Here, a series of new methods are presented based on the use of a low X-ray-background film as a crystallization support and a photoablation laser that enable the automation of major operations required for the preparation of crystals for X-ray diffraction experiments. In this approach, the controlled removal of the mother liquor before crystal mounting simplifies the cryocooling process, in many cases eliminating the use of cryoprotectant agents, while crystal-soaking experiments are performed through diffusion, precluding the need for repeated sample-recovery and transfer operations. Moreover, the high-precision laser enables new mounting strategies that are not accessible through other methods. This approach bridges an important gap in automation and can contribute to expanding the capabilities of modern macromolecular crystallography facilities.
PDB ID: 5AMWDownload
MMDB ID: 138328
PDB Deposition Date: 2015/9/2
Updated in MMDB: 2016/05
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 5AMW: monomeric; determined by author and by software (PISA)
Molecular Components in 5AMW
Label Count Molecule
Protein (1 molecule)
1
FRA a 2 Allergen
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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