5ADY: Cryo-EM structures of the 50S ribosome subunit bound with HflX

Citation:
Abstract
Adverse cellular conditions often lead to nonproductive translational stalling and arrest of ribosomes on mRNAs. Here, we used fast kinetics and cryo-EM to characterize Escherichia coli HflX, a GTPase with unknown function. Our data reveal that HflX is a heat shock-induced ribosome-splitting factor capable of dissociating vacant as well as mRNA-associated ribosomes with deacylated tRNA in the peptidyl site. Structural data demonstrate that the N-terminal effector domain of HflX binds to the peptidyl transferase center in a strikingly similar manner as that of the class I release factors and induces dramatic conformational changes in central intersubunit bridges, thus promoting subunit dissociation. Accordingly, loss of HflX results in an increase in stalled ribosomes upon heat shock. These results suggest a primary role of HflX in rescuing translationally arrested ribosomes under stress conditions.
PDB ID: 5ADYDownload
MMDB ID: 133322
PDB Deposition Date: 2015/8/25
Updated in MMDB: 2015/12
Experimental Method:
electron microscopy
Resolution: 4.5  Å
Source Organism:
Escherichia coli K-12
Similar Structures:
Biological Unit for 5ADY: 34-meric; determined by author
Molecular Components in 5ADY
Label Count Molecule
Proteins (32 molecules)
1
50S Ribosomal Protein L32(Gene symbol: rpmF)
Molecule annotation
1
50S Ribosomal Protein L33(Gene symbol: rpmG)
Molecule annotation
1
50S Ribosomal Protein L34(Gene symbol: rpmH)
Molecule annotation
1
50S Ribosomal Protein L35(Gene symbol: rpmI)
Molecule annotation
1
50S Ribosomal Protein L36(Gene symbol: rpmJ)
Molecule annotation
1
50S Ribosomal Protein L1(Gene symbol: rplA)
Molecule annotation
1
Gtpase Hflx(Gene symbol: hflX)
Molecule annotation
1
50S Ribosomal Protein L10(Gene symbol: rplJ)
Molecule annotation
1
50S Ribosomal Protein L2(Gene symbol: rplB)
Molecule annotation
1
50S Ribosomal Protein L3(Gene symbol: rplC)
Molecule annotation
1
50S Ribosomal Protein L4(Gene symbol: rplD)
Molecule annotation
1
50S Ribosomal Protein L5(Gene symbol: rplE)
Molecule annotation
1
50S Ribosomal Protein L6(Gene symbol: rplF)
Molecule annotation
1
50S Ribosomal Protein L9(Gene symbol: rplI)
Molecule annotation
1
50S Ribosomal Protein L11(Gene symbol: rplK)
Molecule annotation
1
50S Ribosomal Protein L13(Gene symbol: rplM)
Molecule annotation
1
50S Ribosomal Protein L14(Gene symbol: rplN)
Molecule annotation
1
50S Ribosomal Protein L15(Gene symbol: rplO)
Molecule annotation
1
50S Ribosomal Protein L16(Gene symbol: rplP)
Molecule annotation
1
50S Ribosomal Protein L17(Gene symbol: rplQ)
Molecule annotation
1
50S Ribosomal Protein L18(Gene symbol: rplR)
Molecule annotation
1
50S Ribosomal Protein L19(Gene symbol: rplS)
Molecule annotation
1
50S Ribosomal Protein L20(Gene symbol: rplT)
Molecule annotation
1
50S Ribosomal Protein L21(Gene symbol: rplU)
Molecule annotation
1
50S Ribosomal Protein L22(Gene symbol: rplV)
Molecule annotation
1
50S Ribosomal Protein L23(Gene symbol: rplW)
Molecule annotation
1
50S Ribosomal Protein L24(Gene symbol: rplX)
Molecule annotation
1
50S Ribosomal Protein L25(Gene symbol: rplY)
Molecule annotation
1
50S Ribosomal Protein L27(Gene symbol: rpmA)
Molecule annotation
1
50S Ribosomal Protein L28(Gene symbol: rpmB)
Molecule annotation
1
50S Ribosomal Protein L29(Gene symbol: rpmC)
Molecule annotation
1
50S Ribosomal Protein L30(Gene symbol: rpmD)
Molecule annotation
Nucleotides(2 molecules)
1
5S rRNA
Molecule annotation
1
23S rRNA
Molecule annotation
Chemicals (2 molecules)
1
1
2
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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