5ADX: Cryoem Structure Of Dynactin Complex At 4.0 Angstrom Resolution

Citation:
Abstract
Dynactin is an essential cofactor for the microtubule motor cytoplasmic dynein-1. We report the structure of the 23-subunit dynactin complex by cryo-electron microscopy to 4.0 angstroms. Our reconstruction reveals how dynactin is built around a filament containing eight copies of the actin-related protein Arp1 and one of beta-actin. The filament is capped at each end by distinct protein complexes, and its length is defined by elongated peptides that emerge from the alpha-helical shoulder domain. A further 8.2 angstrom structure of the complex between dynein, dynactin, and the motility-inducing cargo adaptor Bicaudal-D2 shows how the translational symmetry of the dynein tail matches that of the dynactin filament. The Bicaudal-D2 coiled coil runs between dynein and dynactin to stabilize the mutually dependent interactions between all three components.
PDB ID: 5ADXDownload
MMDB ID: 135162
PDB Deposition Date: 2015/8/24
Updated in MMDB: 2015/12
Experimental Method:
electron microscopy
Resolution: 4  Å
Source Organism:
Similar Structures:
Biological Unit for 5ADX: 27-meric; determined by author
Molecular Components in 5ADX
Label Count Molecule
Proteins (27 molecules)
8
Actin Related Protein 1
Molecule annotation
1
Actin, Cytoplasmic 1(Gene symbol: ACTB)
Molecule annotation
1
Actin Related Protein 11
Molecule annotation
1
Capping Protein (Actin Filament) Muscle Z-line, Alpha 1
Molecule annotation
1
F-actin Capping Protein Beta Subunit Variant II
Molecule annotation
1
Dynactin Subunit 2
Molecule annotation
1
Dynactin Subunit 2
Molecule annotation
2
Dynactin Subunit 3
Molecule annotation
2
Dynactin Subunit 2
Molecule annotation
1
Dynactin 5
Molecule annotation
1
Dynactin Subunit 6
Molecule annotation
1
Dynactin Subunit 2
Molecule annotation
2
Dynactin Subunit 2
Molecule annotation
1
Dynactin Subunit 2
Molecule annotation
1
Dynactin Subunit 2
Molecule annotation
1
Dynactin Subunit 2
Molecule annotation
1
Dynactin Subunit 2
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.