5ACH: X-ray Structure of LPMO

Citation:
Abstract
Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization of biomass, especially in the sustainable generation of cellulosic bioethanol. We report here a structural determination of an LPMO-oligosaccharide complex, yielding detailed insights into the mechanism of action of these enzymes. Using a combination of structure and electron paramagnetic resonance spectroscopy, we reveal the means by which LPMOs interact with saccharide substrates. We further uncover electronic and structural features of the enzyme active site, showing how LPMOs orchestrate the reaction of oxygen with polysaccharide chains.
PDB ID: 5ACHDownload
MMDB ID: 136935
PDB Deposition Date: 2015/8/17
Updated in MMDB: 2016/04
Experimental Method:
x-ray diffraction
Resolution: 1.28  Å
Source Organism:
Similar Structures:
Biological Unit for 5ACH: monomeric; determined by author and by software (PISA)
Molecular Components in 5ACH
Label Count Molecule
Protein (1 molecule)
1
Lytic Polysaccharide Monooxygenase
Molecule annotation
Chemicals (17 molecules)
1
15
2
1
3
1
* Click molecule labels to explore molecular sequence information.

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