5A8U: Crystal Structure Of Orgyia Pseudotsugata Cpv5 Polyhedra

Citation:
Abstract
Polyhedra represent an ancient system used by a number of insect viruses to protect virions during long periods of environmental exposure. We present high resolution crystal structures of polyhedra for seven previously uncharacterised types of cypoviruses, four using ab initio selenomethionine phasing (two of these required over 100 selenomethionine crystals each). Approximately 80% of residues are structurally equivalent between all polyhedrins (pairwise rmsd 1.5A), whilst pairwise sequence identities, based on structural alignment, are as little as 12%. These structures illustrate the effect of 400million years of evolution on a system where the crystal lattice is the functionally conserved feature in the face of massive sequence variability. The conservation of crystal contacts is maintained across most of the molecular surface, except for a dispensable virus recognition domain. By spreading the contacts over so much of the protein surface the lattice remains robust in the face of many individual changes. Overall these unusual structural constraints seem to have skewed the molecule's evolution so that surface residues are almost as conserved as the internal residues.
PDB ID: 5A8UDownload
MMDB ID: 132264
PDB Deposition Date: 2015/7/17
Updated in MMDB: 2015/11
Experimental Method:
x-ray diffraction
Resolution: 1.61  Å
Source Organism:
Similar Structures:
Biological Unit for 5A8U: trimeric; determined by software (PISA)
Molecular Components in 5A8U
Label Count Molecule
Proteins (3 molecules)
3
Polyhedrin
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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