5A4W: Atgstf2 From Arabidopsis Thaliana In Complex With Quercetrin

Glutathione transferases (GSTs) are involved in many processes in plant biochemistry, with their best characterised role being the detoxification of xenobiotics through their conjugation with glutathione. GSTs have also been implicated in noncatalytic roles, including the binding and transport of small heterocyclic ligands such as indole hormones, phytoalexins and flavonoids. Although evidence for ligand binding and transport has been obtained using gene deletions and ligand binding studies on purified GSTs, there has been no structural evidence for the binding of relevant ligands in noncatalytic sites. Here we provide evidence of noncatalytic ligand-binding sites in the phi class GST from the model plant Arabidopsis thaliana, AtGSTF2, revealed by X-ray crystallography. Complexes of the AtGSTF2 dimer were obtained with indole-3-aldehyde, camalexin, the flavonoid quercetrin and its non-rhamnosylated analogue quercetin, at resolutions of 2.00, 2.77, 2.25 and 2.38 A respectively. Two symmetry-equivalent-binding sites (L1) were identified at the periphery of the dimer, and one more (L2) at the dimer interface. In the complexes, indole-3-aldehyde and quercetrin were found at both L1 and L2 sites, but camalexin was found only at the L1 sites and quercetin only at the L2 site. Ligand binding at each site appeared to be largely determined through hydrophobic interactions. The crystallographic studies support previous conclusions made on ligand binding in noncatalytic sites by AtGSTF2 based on isothermal calorimetry experiments (Dixon et al. (2011) Biochem J438, 63-70) and suggest a mode of ligand binding in GSTs commensurate with a possible role in ligand transport.
PDB ID: 5A4WDownload
MMDB ID: 140436
PDB Deposition Date: 2015/6/15
Updated in MMDB: 2016/07
Experimental Method:
x-ray diffraction
Resolution: 2.25  Å
Similar Structures:
Biological Unit for 5A4W: hexameric; determined by author and by software (PISA)
Molecular Components in 5A4W
Label Count Molecule
Proteins (6 molecules)
Glutathione S-transferase F2(Gene symbol: GSTF2)
Molecule annotation
Chemicals (14 molecules)
* Click molecule labels to explore molecular sequence information.

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