5A2H: Crystal Structure Of Arabidopsis Thaliana Calmodulin-7

Citation:
Abstract
Calmodulin (CaM) is a Ca(2+) sensor that participates in several cellular signaling cascades by interacting with various targets, including DNA. It has been shown that Arabidopsis thaliana CaM7 (AtCaM7) interacts with Z-box DNA and functions as a transcription factor [Kushwaha R et al. (2008) Plant Cell 20, 1747-1759; Abbas N et al. (2014) Plant Cell 26, 1036-1052]. The crystal structure of AtCaM7, and a model of the AtCAM7-Z-box complex suggest that Arg-127 determines the DNA-binding ability by forming crucial interactions with the guanine base. We validated the model using biolayer interferometry, which confirmed that AtCaM7 interacts with Z-box DNA with high affinity. In contrast, the AtCaM2/3/5 isoform does not show any binding, although it differs from AtCaM7 by only a single residue.
PDB ID: 5A2HDownload
MMDB ID: 137852
PDB Deposition Date: 2015/5/19
Updated in MMDB: 2016/09
Experimental Method:
x-ray diffraction
Resolution: 2.27  Å
Source Organism:
Similar Structures:
Biological Unit for 5A2H: monomeric; determined by author and by software (PISA)
Molecular Components in 5A2H
Label Count Molecule
Protein (1 molecule)
1
Calmodulin
Molecule annotation
Chemicals (8 molecules)
1
4
2
3
3
1
* Click molecule labels to explore molecular sequence information.

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