5A1Y: The Structure Of The Copi Coat Linkage Iv

Citation:
Abstract
Transport of material within cells is mediated by trafficking vesicles that bud from one cellular compartment and fuse with another. Formation of a trafficking vesicle is driven by membrane coats that localize cargo and polymerize into cages to bend the membrane. Although extensive structural information is available for components of these coats, the heterogeneity of trafficking vesicles has prevented an understanding of how complete membrane coats assemble on the membrane. We combined cryo-electron tomography, subtomogram averaging, and cross-linking mass spectrometry to derive a complete model of the assembled coat protein complex I (COPI) coat involved in traffic between the Golgi and the endoplasmic reticulum. The highly interconnected COPI coat structure contradicted the current "adaptor-and-cage" understanding of coated vesicle formation.
PDB ID: 5A1YDownload
MMDB ID: 154016
PDB Deposition Date: 2015/5/6
Updated in MMDB: 2017/09
Experimental Method:
electron microscopy
Resolution: 21  Å
Source Organism:
Mus musculus
Similar Structures:
Biological Unit for 5A1Y: 21-meric; determined by software (PISA)
Molecular Components in 5A1Y
Label Count Molecule
Proteins (21 molecules)
5
Adp-ribosylation Factor 1(Gene symbol: ARF1)
Molecule annotation
2
Coatomer Subunit Alpha(Gene symbol: Copa)
Molecule annotation
2
Coatomer Subunit Beta'(Gene symbol: Copb2)
Molecule annotation
3
Coatomer Subunit Gamma-1(Gene symbol: Copg1)
Molecule annotation
3
Coatomer Subunit Zeta-1(Gene symbol: Copz1)
Molecule annotation
2
Coatomer Subunit Beta(Gene symbol: Copb1)
Molecule annotation
2
Coatomer Subunit Delta(Gene symbol: Arcn1)
Molecule annotation
2
Coatomer Subunit Epsilon(Gene symbol: Cope)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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