4ZT0: Crystal Structure Of Catalytically-active Streptococcus Pyogenes Cas9 In Complex With Single-guide Rna At 2.9 Angstrom Resolution

Citation:
Abstract
Bacterial adaptive immunity uses CRISPR (clustered regularly interspaced short palindromic repeats)-associated (Cas) proteins together with CRISPR transcripts for foreign DNA degradation. In type II CRISPR-Cas systems, activation of Cas9 endonuclease for DNA recognition upon guide RNA binding occurs by an unknown mechanism. Crystal structures of Cas9 bound to single-guide RNA reveal a conformation distinct from both the apo and DNA-bound states, in which the 10-nucleotide RNA "seed" sequence required for initial DNA interrogation is preordered in an A-form conformation. This segment of the guide RNA is essential for Cas9 to form a DNA recognition-competent structure that is poised to engage double-stranded DNA target sequences. We construe this as convergent evolution of a "seed" mechanism reminiscent of that used by Argonaute proteins during RNA interference in eukaryotes.
PDB ID: 4ZT0Download
MMDB ID: 130731
PDB Deposition Date: 2015/5/14
Updated in MMDB: 2015/07
Experimental Method:
x-ray diffraction
Resolution: 2.9  Å
Source Organism:
Similar Structures:
Biological Unit for 4ZT0: dimeric; determined by author and by software (PISA)
Molecular Components in 4ZT0
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease Cas9
Molecule annotation
Nucleotide(1 molecule)
1
Single-guide RNA
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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