4ZKT: Crystal Structure Of The Progenitor M Complex Of Clostridium Botulinum Type E Neurotoxin

Citation:
Abstract
Clostridium botulinum neurotoxin (BoNT) is released as a progenitor complex, in association with a non-toxic-non-hemagglutinin protein (NTNH) and other associated proteins. We have determined the crystal structure of M type Progenitor complex of botulinum neurotoxin E [PTC-E(M)], a heterodimer of BoNT and NTNH. The crystal structure reveals that the complex exists as a tight, interlocked heterodimer of BoNT and NTNH. The crystal structure explains the mechanism of molecular assembly of the complex and reveals several acidic clusters at the interface responsible for association at low acidic pH and disassociation at basic/neutral pH. The similarity of the general architecture between the PTC-E(M) and the previously determined PTC-A(M) strongly suggests that the progenitor M complexes of all botulinum serotypes may have similar molecular arrangement, although the neurotoxins apparently can take very different conformation when they are released from the M complex.
PDB ID: 4ZKTDownload
MMDB ID: 135017
PDB Deposition Date: 2015/4/30
Updated in MMDB: 2015/12
Experimental Method:
x-ray diffraction
Resolution: 3.05  Å
Source Organism:
Similar Structures:
Biological Unit for 4ZKT: dimeric; determined by author and by software (PISA)
Molecular Components in 4ZKT
Label Count Molecule
Proteins (2 molecules)
1
Bontoxilysin a
Molecule annotation
1
Botulinum Neurotoxin Type E, Nontoxic-nonhemagglutinin Component, Ntnh
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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