4ZBY: Family 4 Uracil-dna Glycosylase From Sulfolobus Tokodaii (uracil Complex Form)

Citation:
Abstract
Uracil-DNA glycosylases (UDGs) excise uracil from DNA by catalyzing the N-glycosidic bond hydrolysis. Here we report the first crystal structures of an archaeal UDG (stoUDG). Compared with other UDGs, stoUDG has a different structure of the leucine-intercalation loop, which is important for DNA binding. The stoUDG-DNA complex model indicated that Leu169, Tyr170, and Asn171 in the loop are involved in DNA intercalation. Mutational analysis showed that Tyr170 is critical for substrate DNA recognition. These results indicate that Tyr170 occupies the intercalation site formed after the structural change of the leucine-intercalation loop required for the catalysis.
PDB ID: 4ZBYDownload
MMDB ID: 132439
PDB Deposition Date: 2015/4/15
Updated in MMDB: 2015/09
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
Similar Structures:
Biological Unit for 4ZBY: monomeric; determined by author and by software (PISA)
Molecular Components in 4ZBY
Label Count Molecule
Protein (1 molecule)
1
Uracil-dna Glycosylase(Gene symbol: STK_RS12375)
Molecule annotation
Chemicals (3 molecules)
1
1
2
1
3
1
* Click molecule labels to explore molecular sequence information.

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