4Z66: Nucleosome Disassembly By Rsc And Swi/snf Is Enhanced By H3 Acetylation Near The Nucleosome Dyad Axis

Citation:
Abstract
Signaling associated with transcription activation occurs through posttranslational modification of histones and is best exemplified by lysine acetylation. Lysines are acetylated in histone tails and the core domain/lateral surface of histone octamers. While acetylated lysines in histone tails are frequently recognized by other factors referred to as "readers," which promote transcription, the mechanistic role of the modifications in the lateral surface of the histone octamer remains unclear. By using X-ray crystallography, we found that acetylated lysines 115 and 122 in histone H3 are solvent accessible, but in biochemical assays they appear not to interact with the bromodomains of SWI/SNF and RSC to enhance recruitment or nucleosome mobilization, as previously shown for acetylated lysines in H3 histone tails. Instead, we found that acetylation of lysines 115 and 122 increases the predisposition of nucleosomes for disassembly by SWI/SNF and RSC up to 7-fold, independent of bromodomains, and only in conjunction with contiguous nucleosomes. Thus, in combination with SWI/SNF and RSC, acetylation of lateral surface lysines in the histone octamer serves as a crucial regulator of nucleosomal dynamics distinct from the histone code readers and writers.
PDB ID: 4Z66Download
MMDB ID: 133287
PDB Deposition Date: 2015/4/3
Updated in MMDB: 2015/11
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 4Z66: decameric; determined by author and by software (PISA)
Molecular Components in 4Z66
Label Count Molecule
Proteins (8 molecules)
2
Histone H3.2(Gene symbol: hist2h3c.L)
Molecule annotation
2
Histone H4(Gene symbol: hist1h4a.L)
Molecule annotation
2
Histone H2A
Molecule annotation
2
Histone H2B 1.1(Gene symbol: hist1h2bj.S)
Molecule annotation
Nucleotides(2 molecules)
1
DNA (147-mer)
Molecule annotation
1
DNA (147-mer)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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