4YXL: Crystal Structure Of Syrian Hamster Prion Protein Complexed With Pom1 Fab

Misfolded prion proteins are the cause of neurodegenerative diseases that affect many mammalian species, including humans. Transmission of the prion diseases poses a considerable public-health risk as a specific prion disease such as bovine spongiform encephalopathy can be transferred to humans and other mammalian species upon contaminant exposure. The underlying mechanism of prion propagation and the species barriers that control cross species transmission has been investigated quite extensively. So far a number of prion strains have been characterized and those have been intimately linked to species-specific infectivity and other pathophysiological manifestations. These strains are encoded by a protein-only agent, and have a high degree of sequence identity across mammalian species. The molecular events that lead to strain differentiation remain elusive. In order to contribute to the understanding of strain differentiation, we have determined the crystal structures of the globular, folded domains of four prion proteins (cow, deer, elk and Syrian hamster) bound to the POM1 antibody fragment Fab. Although the overall structural folds of the mammalian prion proteins remains extremely similar, there are several local structural variations observed in the misfolding-initiator motifs. In additional molecular dynamics simulation studies on these several prion proteins reveal differences in the local fluctuations and imply that these differences have possible roles in the unfolding of the globular domains. These local variations in the structured domains perpetuate diverse patterns of prion misfolding and possibly facilitate the strain selection and adaptation.
PDB ID: 4YXLDownload
MMDB ID: 132856
PDB Deposition Date: 2015/3/23
Updated in MMDB: 2015/11
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
Mus musculus
Similar Structures:
Biological Unit for 4YXL: monomeric; determined by author and by software (PISA)
Molecular Components in 4YXL
Label Count Molecule
Protein (1 molecule)
Major Prion Protein(Gene symbol: Prnp)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB