4YSQ: Structure Of Copper Nitrite Reductase From Geobacillus Thermodenitrificans - 8.38 Mgy

Citation:
Abstract
Serial femtosecond crystallography (SFX) has enabled the damage-free structural determination of metalloenzymes and filled the gaps of our knowledge between crystallographic and spectroscopic data. Crystallographers, however, scarcely know whether the rising technique provides truly new structural insights into mechanisms of metalloenzymes partly because of limited resolutions. Copper nitrite reductase (CuNiR), which converts nitrite to nitric oxide in denitrification, has been extensively studied by synchrotron radiation crystallography (SRX). Although catalytic Cu (Type 2 copper (T2Cu)) of CuNiR had been suspected to tolerate X-ray photoreduction, we here showed that T2Cu in the form free of nitrite is reduced and changes its coordination structure in SRX. Moreover, we determined the completely oxidized CuNiR structure at 1.43 A resolution with SFX. Comparison between the high-resolution SFX and SRX data revealed the subtle structural change of a catalytic His residue by X-ray photoreduction. This finding, which SRX has failed to uncover, provides new insight into the reaction mechanism of CuNiR.
PDB ID: 4YSQDownload
MMDB ID: 136691
PDB Deposition Date: 2015/3/17
Updated in MMDB: 2016/05
Experimental Method:
x-ray diffraction
Resolution: 1.5  Å
Source Organism:
Similar Structures:
Biological Unit for 4YSQ: trimeric; determined by author and by software (PISA)
Molecular Components in 4YSQ
Label Count Molecule
Proteins (3 molecules)
3
Nitrite Reductase
Molecule annotation
Chemicals (27 molecules)
1
21
2
3
3
3
* Click molecule labels to explore molecular sequence information.

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