4YK8: Crystal Structure Of The Atg101-atg13 Complex From Fission Yeast

Atg101 is an essential component of the autophagy-initiating ULK complex in higher eukaryotes, but it is absent from the functionally equivalent Atg1 complex in budding yeast. Here, we report the crystal structure of the fission yeast Atg101-Atg13 complex. Atg101 has a Hop1, Rev7 and Mad2 (HORMA) architecture similar to that of Atg13. Mad2 HORMA has two distinct conformations (O-Mad2 and C-Mad2), and, intriguingly, Atg101 resembles O-Mad2 rather than the C-Mad2-like Atg13. Atg13 HORMA from higher eukaryotes possesses an inherently unstable fold, which is stabilized by Atg101 via interactions analogous to those between O-Mad2 and C-Mad2. Mutational studies revealed that Atg101 is responsible for recruiting downstream factors to the autophagosome-formation site in mammals via a newly identified WF finger. These data define the molecular functions of Atg101, providing a basis for elucidating the molecular mechanisms of mammalian autophagy initiation by the ULK complex.
PDB ID: 4YK8Download
MMDB ID: 129776
PDB Deposition Date: 2015/3/4
Updated in MMDB: 2015/07
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Similar Structures:
Biological Unit for 4YK8: dimeric; determined by author and by software (PISA)
Molecular Components in 4YK8
Label Count Molecule
Proteins (2 molecules)
Meiotically Up-regulated Gene 66 Protein(Gene symbol: mug66)
Molecule annotation
Autophagy Protein 13(Gene symbol: atg13)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB