4YK4: Human Antibody 641 I-9 In Complex With Influenza Hemagglutinin H1 Solomon Islands/03/2006

Citation:
Abstract
Vaccines for rapidly evolving pathogens will confer lasting immunity if they elicit antibodies recognizing conserved epitopes, such as a receptor-binding site (RBS). From characteristics of an influenza-virus RBS-directed antibody, we devised a signature motif to search for similar antibodies. We identified, from three vaccinees, over 100 candidates encoded by 11 different VH genes. Crystal structures show that antibodies in this class engage the hemagglutinin RBS and mimic binding of the receptor, sialic acid, by supplying a critical dipeptide on their projecting, heavy-chain third complementarity determining region. They share contacts with conserved, receptor-binding residues but contact different residues on the RBS periphery, limiting the likelihood of viral escape when several such antibodies are present. These data show that related modes of RBS recognition can arise from different germline origins and mature through diverse affinity maturation pathways. Immunogens focused on an RBS-directed response will thus have a broad range of B cell targets.
PDB ID: 4YK4Download
MMDB ID: 129619
PDB Deposition Date: 2015/3/3
Updated in MMDB: 2017/09
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Influenza A virus (A/Solomon Islands/3/2006(H1N1))
Similar Structures:
Biological Unit for 4YK4: trimeric; determined by author
Molecular Components in 4YK4
Label Count Molecule
Proteins (3 molecules)
1
641 I-9 Vlcl Antibody
Molecule annotation
1
641 I-9 Vhch Antibody
Molecule annotation
1
Hemagglutinin
Molecule annotation
Chemicals (4 molecules)
1
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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