4YHH: Crystal Structure Of The 30s Ribosomal Subunit From Thermus Thermophilus In Complex With Tigecycline

Citation:
Abstract
Although both tetracycline and tigecycline inhibit protein synthesis by sterically hindering the binding of tRNA to the ribosomal A site, tigecycline shows increased efficacy in both in vitro and in vivo activity assays and escapes the most common resistance mechanisms associated with the tetracycline class of antibiotics. These differences in activities are attributed to the tert-butyl-glycylamido side chain found in tigecycline. Our structural analysis by X-ray crystallography shows that tigecycline binds the bacterial 30S ribosomal subunit with its tail in an extended conformation and makes extensive interactions with the 16S rRNA nucleotide C1054. These interactions restrict the mobility of C1054 and contribute to the antimicrobial activity of tigecycline, including its resistance to the ribosomal protection proteins.
PDB ID: 4YHHDownload
MMDB ID: 128489
PDB Deposition Date: 2015/2/27
Updated in MMDB: 2015/04
Experimental Method:
x-ray diffraction
Resolution: 3.42  Å
Source Organism:
Similar Structures:
Biological Unit for 4YHH: 21-meric; determined by software (PISA)
Molecular Components in 4YHH
Label Count Molecule
Proteins (20 molecules)
1
30S Ribosomal Protein S2(Gene symbol: rpsB)
Molecule annotation
1
30S Ribosomal Protein S3(Gene symbol: rpsC)
Molecule annotation
1
30S Ribosomal Protein S4(Gene symbol: rpsD)
Molecule annotation
1
30S Ribosomal Protein S5(Gene symbol: rpsE)
Molecule annotation
1
30S Ribosomal Protein S6(Gene symbol: rpsF)
Molecule annotation
1
30S Ribosomal Protein S7(Gene symbol: TTHA1696)
Molecule annotation
1
30S Ribosomal Protein S8
Molecule annotation
1
30S Ribosomal Protein S9(Gene symbol: rpsI)
Molecule annotation
1
30S Ribosomal Protein S10(Gene symbol: rpsJ)
Molecule annotation
1
30S Ribosomal Protein S11(Gene symbol: TTHA1666)
Molecule annotation
1
30S Ribosomal Protein S12(Gene symbol: rpsL)
Molecule annotation
1
30S Ribosomal Protein S13(Gene symbol: rpsM)
Molecule annotation
1
30S Ribosomal Protein S14 Type Z
Molecule annotation
1
30S Ribosomal Protein S15(Gene symbol: rpsO)
Molecule annotation
1
30S Ribosomal Protein S16(Gene symbol: rpsP)
Molecule annotation
1
30S Ribosomal Protein S17
Molecule annotation
1
30S Ribosomal Protein S18(Gene symbol: rpsR)
Molecule annotation
1
30S Ribosomal Protein S19(Gene symbol: rpsS)
Molecule annotation
1
30S Ribosomal Protein S20(Gene symbol: rpsT)
Molecule annotation
1
30S Ribosomal Protein THX(Gene symbol: TTHA1396)
Molecule annotation
Nucleotide(1 molecule)
1
16S Ribosomal RNA
Molecule annotation
Chemicals (106 molecules)
1
1
2
103
3
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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