4YGG: Crystal Structure Of R111k:y134f:t54v:r132q:p39y:r59y Mutant Of Human Cellular Retinoic Acid Binding Protein Ii With Retinal At 1.9 Angstrom Resolution - Visible Light Irradiated Crystal - 2nd Cycle

The members of the rhodopsin family of proteins are involved in many essential light-dependent processes in biology. Specific photoisomerization of the protein-bound retinylidene PSB at a specified wavelength range of light is at the heart of all of these systems. Nonetheless, it has been difficult to reproduce in an engineered system. We have developed rhodopsin mimics, using intracellular lipid binding protein family members as scaffolds, to study fundamental aspects of protein/chromophore interactions. Herein we describe a system that specifically isomerizes the retinylidene protonated Schiff base both thermally and photochemically. This isomerization has been characterized at atomic resolution by quantitatively interconverting the isomers in the crystal both thermally and photochemically. This event is accompanied by a large pKa change of the imine similar to the pKa changes observed in bacteriorhodopsin and visual opsins during isomerization.
PDB ID: 4YGGDownload
MMDB ID: 136902
PDB Deposition Date: 2015/2/26
Updated in MMDB: 2016/08
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 4YGG: monomeric; determined by author
Molecular Components in 4YGG
Label Count Molecule
Protein (1 molecule)
Cellular Retinoic Acid-binding Protein 2(Gene symbol: CRABP2)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

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