4YCX: Binary Complex Of Human Dna Polymerase Mu With 2-nt Gapped Dna Substrate

Citation:
Abstract
Among the many proteins used to repair DNA double-strand breaks by nonhomologous end joining (NHEJ) are two related family X DNA polymerases, Pol lambda and Pol micro. Which of these two polymerases is preferentially used for filling DNA gaps during NHEJ partly depends on sequence complementarity at the break, with Pol lambda and Pol micro repairing complementary and noncomplementary ends, respectively. To better understand these substrate preferences, we present crystal structures of Pol micro on a 2-nt gapped DNA substrate, representing three steps of the catalytic cycle. In striking contrast to Pol lambda, Pol micro "skips" the first available template nucleotide, instead using the template base at the 5' end of the gap to direct nucleotide binding and incorporation. This remarkable divergence from canonical 3'-end gap filling is consistent with data on end-joining substrate specificity in cells, and provides insights into polymerase substrate choices during NHEJ.
PDB ID: 4YCXDownload
MMDB ID: 131455
PDB Deposition Date: 2015/2/20
Updated in MMDB: 2017/10
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 4YCX: tetrameric; determined by author
Molecular Components in 4YCX
Label Count Molecule
Protein (1 molecule)
1
DNA-directed Dna/rna Polymerase MU(Gene symbol: POLM)
Molecule annotation
Nucleotides(3 molecules)
1
DNA (5'-d(*cp*gp*gp*cp*ap*ap*tp*ap*cp*g)-3')
Molecule annotation
1
DNA (5'-d(*cp*gp*tp*a)-3')
Molecule annotation
1
DNA (5'-d(p*gp*cp*cp*g)-3')
Molecule annotation
Chemicals (6 molecules)
1
1
2
1
3
3
4
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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