4YCR: Structure Determination Of An Integral Membrane Protein At Room Temperature From Crystals In Situ

Citation:
Abstract
The structure determination of an integral membrane protein using synchrotron X-ray diffraction data collected at room temperature directly in vapour-diffusion crystallization plates (in situ) is demonstrated. Exposing the crystals in situ eliminates manual sample handling and, since it is performed at room temperature, removes the complication of cryoprotection and potential structural anomalies induced by sample cryocooling. Essential to the method is the ability to limit radiation damage by recording a small amount of data per sample from many samples and subsequently assembling the resulting data sets using specialized software. The validity of this procedure is established by the structure determination of Haemophilus influenza TehA at 2.3 A resolution. The method presented offers an effective protocol for the fast and efficient determination of membrane-protein structures at room temperature using third-generation synchrotron beamlines.
PDB ID: 4YCRDownload
MMDB ID: 129770
PDB Deposition Date: 2015/2/20
Updated in MMDB: 2015/06
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 4YCR: trimeric; determined by author and by software (PISA)
Molecular Components in 4YCR
Label Count Molecule
Proteins (3 molecules)
3
Tellurite Resistance Protein Teha Homolog(Gene symbol: HI0511)
Molecule annotation
Chemicals (6 molecules)
1
6
* Click molecule labels to explore molecular sequence information.

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