4YC7: Crystal Structure Of Human Fmnl2 Gbd-fh3 Domains Bound To Cdc42-gppnhp

Formins are actin polymerization factors that elongate unbranched actin filaments at the barbed end. Rho family GTPases activate Diaphanous-related formins through the relief of an autoregulatory interaction. The crystal structures of the N-terminal domains of human FMNL1 and FMNL2 in complex with active Cdc42 show that Cdc42 mediates contacts with all five armadillo repeats of the formin with specific interactions formed by the Rho-GTPase insert helix. Mutation of three residues within Rac1 results in a gain-of-function mutation for FMNL2 binding and reconstitution of the Cdc42 phenotype in vivo. Dimerization of FMNL1 through a parallel coiled coil segment leads to formation of an umbrella-shaped structure that-together with Cdc42-spans more than 15 nm in diameter. The two interacting FMNL-Cdc42 heterodimers expose six membrane interaction motifs on a convex protein surface, the assembly of which may facilitate actin filament elongation at the leading edge of lamellipodia and filopodia.
PDB ID: 4YC7Download
MMDB ID: 129279
PDB Deposition Date: 2015/2/19
Updated in MMDB: 2015/05
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Similar Structures:
Biological Unit for 4YC7: dimeric; determined by author and by software (PISA)
Molecular Components in 4YC7
Label Count Molecule
Proteins (2 molecules)
Formin-like Protein 2(Gene symbol: FMNL2)
Molecule annotation
Cell Division Control Protein 42 Homolog(Gene symbol: CDC42)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB