4XWW: Crystal Structure Of Rnase J Complexed With Rna

RNase J is a conserved ribonuclease that belongs to the beta-CASP family of nucleases. It possesses both endo- and exo-ribonuclease activities, which play a key role in pre-rRNA maturation and mRNA decay. Here we report high-resolution crystal structures of Deinococcus radiodurans RNase J complexed with RNA or uridine 5'-monophosphate in the presence of manganese ions. Biochemical and structural studies revealed that RNase J uses zinc ions for two-metal-ion catalysis. One residue conserved among RNase J orthologues (motif B) forms specific electrostatic interactions with the scissile phosphate of the RNA that is critical for the catalysis and product stabilization. The additional manganese ion, which is coordinated by conserved residues at the dimer interface, is critical for RNase J dimerization and exonuclease activity. The structures may also shed light on the mechanism of RNase J exo- and endonucleolytic activity switch.
PDB ID: 4XWWDownload
MMDB ID: 134802
PDB Deposition Date: 2015/1/29
Updated in MMDB: 2015/12
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4XWW: tetrameric; determined by author and by software (PISA)
Molecular Components in 4XWW
Label Count Molecule
Proteins (2 molecules)
Molecule annotation
Nucleotide(1 molecule)
RNA (5'-d(up*up*up*up*up*up*u)-3')
Molecule annotation
Chemicals (8 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB