4WZW: Crystal Structure Of Human Puf-a In Complex With Dna

Pumilio/feminization of XX and XO animals (fem)-3 mRNA-binding factor (PUF) proteins bind sequence specifically to mRNA targets using a single-stranded RNA-binding domain comprising eight Pumilio (PUM) repeats. PUM repeats have now been identified in proteins that function in pre-rRNA processing, including human Puf-A and yeast Puf6. This is a role not previously ascribed to PUF proteins. Here we present crystal structures of human Puf-A that reveal a class of nucleic acid-binding proteins with 11 PUM repeats arranged in an "L"-like shape. In contrast to classical PUF proteins, Puf-A forms sequence-independent interactions with DNA or RNA, mediated by conserved basic residues. We demonstrate that equivalent basic residues in yeast Puf6 are important for RNA binding, pre-rRNA processing, and mRNA localization. Thus, PUM repeats can be assembled into alternative folds that bind to structured nucleic acids in addition to forming canonical eight-repeat crescent-shaped RNA-binding domains found in classical PUF proteins.
PDB ID: 4WZWDownload
MMDB ID: 125853
PDB Deposition Date: 2014/11/20
Updated in MMDB: 2015/01
Experimental Method:
x-ray diffraction
Resolution: 2.95  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4WZW: trimeric; determined by author and by software (PISA)
Molecular Components in 4WZW
Label Count Molecule
Protein (1 molecule)
Pumilio Domain-containing Protein Kiaa0020(Gene symbol: PUM3)
Molecule annotation
Nucleotides(2 molecules)
DNA (5'-d(p*gp*gp*gp*gp*gp*gp*gp*gp*gp*gp*gp*gp*gp*g)-3')
Molecule annotation
DNA (5'- D(p*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*c)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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