4WWU: Structure of Mex67:Mtr2

The Mex67:Mtr2 complex is the principal yeast nuclear export factor for bulk mRNA and also contributes to ribosomal subunit export. Mex67 is a modular protein constructed from four domains (RRM, LRR, NTF2-like and UBA) that have been thought to be joined by flexible linkers like beads on a string, with the RRM and LRR domains binding RNAs and the NTF2-like and UBA domains binding FG-nucleoporins to facilitate movement through nuclear pores. Here, we show that the NTF2-like domain from Saccharomyces cerevisiae Mex67:Mtr2 also contributes to RNA binding. Moreover, the 3.3 A resolution crystal structure of the Mex67(DeltaUBA):Mtr2 complex, supplemented with small angle X-ray scattering data, indicated that the LRR domain has a defined spatial relationship to the Mex67(NTF2L):Mtr2 region. Conversely, the RRM domain and especially the UBA domain are more mobile. The conformation assumed by the LRR and NTF2-like domains results in clusters of positively-charged residues on each becoming arranged to form a continuous interface for binding RNA on the opposite side of the complex to the region that interacts with FG-nucleoporins to facilitate passage through nuclear pores.
PDB ID: 4WWUDownload
MMDB ID: 126758
PDB Deposition Date: 2014/11/12
Updated in MMDB: 2018/07
Experimental Method:
x-ray diffraction
Resolution: 3.301  Å
Source Organism:
Similar Structures:
Biological Unit for 4WWU: hexameric; determined by author and by software
Molecular Components in 4WWU
Label Count Molecule
Proteins (6 molecules)
mRNA Export Factor Mex67(Gene symbol: MEX67)
Molecule annotation
mRNA Transport Regulator Mtr2(Gene symbol: MTR2)
Molecule annotation
Chemicals (7 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB