4WT8: Crystal Structure Of Bactobolin A Bound To 70s Ribosome-trna Complex

Citation:
Abstract
The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-A-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.
PDB ID: 4WT8Download
MMDB ID: 126351
PDB Deposition Date: 2014/10/29
Updated in MMDB: 2015/02
Experimental Method:
x-ray diffraction
Resolution: 3.4  Å
Source Organism:
Similar Structures:
Biological Unit for 4WT8: 22-meric; determined by author and by software (PISA)
Molecular Components in 4WT8
Label Count Molecule
Proteins (20 molecules)
1
30S Ribosomal Protein S2
Molecule annotation
1
30S Ribosomal Protein S3
Molecule annotation
1
30S Ribosomal Protein S4
Molecule annotation
1
30S Ribosomal Protein S5
Molecule annotation
1
30S Ribosomal Protein S6
Molecule annotation
1
30S Ribosomal Protein S7
Molecule annotation
1
30S Ribosomal Protein S8
Molecule annotation
1
30S Ribosomal Protein S9
Molecule annotation
1
30S Ribosomal Protein S10
Molecule annotation
1
30S Ribosomal Protein S11
Molecule annotation
1
30S Ribosomal Protein S12
Molecule annotation
1
30S Ribosomal Protein S13
Molecule annotation
1
30S Ribosomal Protein S14 Type Z
Molecule annotation
1
30S Ribosomal Protein S15
Molecule annotation
1
30S Ribosomal Protein S16
Molecule annotation
1
30S Ribosomal Protein S17
Molecule annotation
1
30S Ribosomal Protein S18
Molecule annotation
1
30S Ribosomal Protein S19
Molecule annotation
1
30S Ribosomal Protein S20
Molecule annotation
1
30S Ribosomal Protein THX
Molecule annotation
Nucleotides(2 molecules)
1
mRNA
Molecule annotation
1
RNA (1504-mer)
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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