National Center for
4WT8: Crystal Structure Of Bactobolin A Bound To 70s Ribosome-trna Complex
J. Mol. Biol. (2015) 427 p.753-755
The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-A-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.