4WPX: Chaetomium theromophilum TREX2 CID domain complex

Citation:
Abstract
The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67:Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium thermophilum TREX-2, show that the in vitro reconstituted complex has an annular structure, and define the structural basis for interactions between Sac3, Sus1, Cdc31, and Mex67:Mtr2. Crystal structures show that the binding of C. thermophilum Sac3N to the Mex67 NTF2-like domain (Mex67(NTF2L)) is mediated primarily through phenylalanine residues present in a series of repeating sequence motifs that resemble those seen in many nucleoporins, and Mlp1 also binds Mex67:Mtr2 using a similar motif. Deletion of Sac3N generated growth and mRNA export defects in Saccharomyces cerevisiae, and we propose TREX-2 and Mlp1 function to facilitate export by concentrating mature messenger ribonucleoparticles at the nuclear pore entrance.
PDB ID: 4WPXDownload
MMDB ID: 130085
PDB Deposition Date: 2014/10/21
Updated in MMDB: 2018/07
Experimental Method:
x-ray diffraction
Resolution: 3.31  Å
Source Organism:
Similar Structures:
Biological Unit for 4WPX: hexameric; determined by software (PISA)
Molecular Components in 4WPX
Label Count Molecule
Proteins (6 molecules)
2
Cell Division Control Protein 31-like Protein
Molecule annotation
2
Putative Sac3 Family Protein
Molecule annotation
2
Putative Uncharacterized Protein
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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