4WP6: Structure of the Mex67 LRR domain from Chaetomium thermophilum

Members of the Mex67-Mtr2/NXF-NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67-Mtr2 that facilitate mRNA nuclear export.
PDB ID: 4WP6Download
MMDB ID: 130891
PDB Deposition Date: 2014/10/17
Updated in MMDB: 2018/07
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
Similar Structures:
Biological Unit for 4WP6: monomeric; determined by author and by software (PISA)
Molecular Components in 4WP6
Label Count Molecule
Protein (1 molecule)
mRNA Export Protein
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB