4WLW: Crystal Structure Of The Ag(i) (activator) Form Of E. Coli Cuer, A Copper Efflux Regulator, Bound To Copa Promoter Dna

Many transcriptional activators act at a distance from core promoter elements and work by recruiting RNA polymerase through protein-protein interactions. We show here how the prokaryotic regulatory protein CueR both represses and activates transcription by differentially modulating local DNA structure within the promoter. Structural studies reveal that the repressor state slightly bends the promoter DNA, precluding optimal RNA polymerase-promoter recognition. Upon binding a metal ion in the allosteric site, CueR switches into an activator conformation. It maintains all protein-DNA contacts but introduces torsional stresses that kink and undertwist the promoter, stabilizing an A-form DNA-like conformation. These factors switch on and off transcription by exerting dynamic control of DNA stereochemistry, reshaping the core promoter and making it a better or worse substrate for polymerase.
PDB ID: 4WLWDownload
MMDB ID: 132187
PDB Deposition Date: 2014/10/8
Updated in MMDB: 2017/10
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Escherichia coli K-12
Similar Structures:
Biological Unit for 4WLW: tetrameric; determined by author
Molecular Components in 4WLW
Label Count Molecule
Proteins (2 molecules)
Hth-type Transcriptional Regulator Cuer(Gene symbol: cueR)
Molecule annotation
Nucleotides(2 molecules)
DNA Non-template Strand (5-d(*dgp*dap*dcp*dcp *dtp*dtp*dcp*dcp*dcp*dcp*dtp*dtp*dgp*dcp*dtp*dgp*dgp*dap *dap*dgp*dgp*dtp*dc)-3
Molecule annotation
DNA Template Strand (5-d(*dgp*dap*dcp*dcp*dtp *dtp*dcp*dcp*dap*dgp*dcp*dap*dap*dgp*dgp*dgp*dgp*dap*dap *dgp*dgp*dtp*dc)-3
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB