4WLA: Time Resolved Serial Femtosecond Crystallography Captures High Resolution Intermediates Of Pyp

Citation:
Abstract
Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.
PDB ID: 4WLADownload
MMDB ID: 125713
PDB Deposition Date: 2014/10/6
Updated in MMDB: 2014/12
Experimental Method:
x-ray diffraction
Resolution: 1.6  Å
Source Organism:
Similar Structures:
Biological Unit for 4WLA: monomeric; determined by author and by software (PISA)
Molecular Components in 4WLA
Label Count Molecule
Protein (1 molecule)
1
Photoactive Yellow Protein
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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