4WJU: Crystal Structure Of Rsa4 From Saccharomyces Cerevisiae

Citation:
Abstract
Eukaryotic ribosome biogenesis involves approximately 200 assembly factors, but how these contribute to ribosome maturation is poorly understood. Here, we identify a network of factors on the nascent 60S subunit that actively remodels preribosome structure. At its hub is Rsa4, a direct substrate of the force-generating ATPase Rea1. We show that Rsa4 is connected to the central protuberance by binding to Rpl5 and to ribosomal RNA (rRNA) helix 89 of the nascent peptidyl transferase center (PTC) through Nsa2. Importantly, Nsa2 binds to helix 89 before relocation of helix 89 to the PTC. Structure-based mutations of these factors reveal the functional importance of their interactions for ribosome assembly. Thus, Rsa4 is held tightly in the preribosome and can serve as a "distribution box," transmitting remodeling energy from Rea1 into the developing ribosome. We suggest that a relay-like factor network coupled to a mechano-enzyme is strategically positioned to relocate rRNA elements during ribosome maturation.
PDB ID: 4WJUDownload
MMDB ID: 124937
PDB Deposition Date: 2014/10/1
Updated in MMDB: 2014/12
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Similar Structures:
Biological Unit for 4WJU: monomeric; determined by author and by software (PISA)
Molecular Components in 4WJU
Label Count Molecule
Protein (1 molecule)
1
Ribosome Assembly Protein 4(Gene symbol: RSA4)
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.