4WJT: Stationary Phase Survival Protein Yuic From B.subtilis Complexed With Nag

Citation:
Abstract
BACKGROUND: Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain. RESULTS: The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA. The YuiC structures are of a domain swapped dimer, although some monomer is also found in solution. The protein crystallised in the presence of pentasaccharide shows a 1,6-anhydrodisaccharide sugar product, indicating that YuiC cleaves the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation. CONCLUSIONS: The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.
PDB ID: 4WJTDownload
MMDB ID: 130692
PDB Deposition Date: 2014/10/1
Updated in MMDB: 2016/09
Experimental Method:
x-ray diffraction
Resolution: 1.21  Å
Source Organism:
Similar Structures:
Biological Unit for 4WJT: dimeric; determined by author and by software (PISA)
Molecular Components in 4WJT
Label Count Molecule
Proteins (2 molecules)
2
Uncharacterized Protein Yuic(Gene symbol: yuiC)
Molecule annotation
Chemicals (7 molecules)
1
2
2
4
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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