4W73: Crystal Structure Of Full-length Split Gfp Mutant E115c/t118h Disulfide Dimer P 21 21 21

Citation:
Abstract
Applications ranging from synthetic biology to protein crystallization could be advanced by facile systems for connecting multiple proteins together in predefined spatial relationships. One approach to this goal is to engineer many distinct assembly forms of a single carrier protein or scaffold, to which other proteins of interest can then be readily attached. In this work we chose GFP as a scaffold and engineered many alternative oligomeric forms, driven by either specific disulfide bond formation or metal ion addition. We generated a wide range of spatial arrangements of GFP subunits from 11 different oligomeric variants, and determined their X-ray structures in a total of 33 distinct crystal forms. Some of the oligomeric GFP variants show geometric polymorphism depending on conditions, while others show considerable geometric rigidity. Potential future applications of this system are discussed.
PDB ID: 4W73Download
MMDB ID: 127160
PDB Deposition Date: 2014/8/21
Updated in MMDB: 2017/10
Experimental Method:
x-ray diffraction
Resolution: 2.79  Å
Source Organism:
Similar Structures:
Biological Unit for 4W73: dimeric; determined by author
Molecular Components in 4W73
Label Count Molecule
Proteins (2 molecules)
2
Fluorescent Protein E115c/t118h
Molecule annotation
Chemicals (2 molecules)
1
1
2
1
* Click molecule labels to explore molecular sequence information.

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